modified pet28a(+) vector (Millipore)
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modified pet28a(+) vector
Modified Pet28a(+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a(+)+vector/pet28a/bio_rxiv__2024__05__15__594396-53-15-17
Average 90 stars, based on 1 article reviews
Modified Pet28a(+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a(+)+vector/pet28a/bio_rxiv__2024__05__15__594396-53-15-17
Average 90 stars, based on 1 article reviews
modified pet28a(+) vector - by Bioz Stars,
2026-10
90/100 stars
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Clone Assay:Article Title: Oligoribonuclease functions as a diribonucleotidase to bypass a key bottleneck in RNA degradation Article Snippet: Orn genes from Vibrio cholerae O1 El Tor VC0341 (residues 1-181) and Homo sapiens REXO2 (residues 33-237) were synthesized by Geneart (Life Technologies). .. Genes were cloned by ligation between BamHI and NotI sites of a modified Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells. Article Snippet: Cloning, expression and purification of E. amylovora DspE and DspE(Δβ-barrel) in Escherichia coli The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_ primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Campylobacter hyointestinalis Isolated from Pigs Produces Multiple Variants of Biologically Active Cytolethal Distending Toxin Article Snippet: The cdt-IIA, cdt-IIB, and cdt-IIC genes (open reading frames [ORFs] 11 to 13 in Fig. 2; also see Table S3 in the supplemental material) were PCR amplified using the genomic DNA of C. hyointestinalis strain ATCC 35217T as the template (see Table S1). .. Each of these amplified DNA fragments was digested with either EcoRI and HindIII or EcoRI and SacI (New England Biolabs Inc.) and cloned into the region downstream of the EcoRI site of the modified Article Title: Structural characterization of NrnC identifies unifying features of dinucleases Article Snippet: For protein expression in Escherichia coli , codon-optimized NrnC genes from B. henselae (BH02530) and B. melitensis (BMEI1828) were synthesized by Geneart (Life Technologies). .. Genes were cloned into a modified Article Title: Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18 Article Snippet: Each of the DNA fragments encoding the human PTPN21 (residues 873-1174, referred to as PTP PTPN21 ) and human PTPN14 (residues 886-1187, referred to as PTP PTPN14 ) PTP domains were cloned into the modified pPRoEX HTa plasmid (Invitrogen, USA) with an N-terminal (His) 10 -linked green fluorescent protein tag and the pET21a plasmid (Novagen, USA), respectively. .. Each of the E7 DNA fragments encoding CR3s of HPV18 (residues 54-105, referred to as CR3 18E7 ), HPV16 (residues 45-98), HPV1a (residues 39-93), and HPV11 (residues 45-98) were cloned into the modified Article Title: Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster Article Snippet: .. The amplified fragments were then cloned into a modified Ligation:Article Title: Oligoribonuclease functions as a diribonucleotidase to bypass a key bottleneck in RNA degradation Article Snippet: Orn genes from Vibrio cholerae O1 El Tor VC0341 (residues 1-181) and Homo sapiens REXO2 (residues 33-237) were synthesized by Geneart (Life Technologies). .. Genes were cloned by ligation between BamHI and NotI sites of a modified Modification:Article Title: Oligoribonuclease functions as a diribonucleotidase to bypass a key bottleneck in RNA degradation Article Snippet: Orn genes from Vibrio cholerae O1 El Tor VC0341 (residues 1-181) and Homo sapiens REXO2 (residues 33-237) were synthesized by Geneart (Life Technologies). .. Genes were cloned by ligation between BamHI and NotI sites of a modified Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells. Article Snippet: Cloning, expression and purification of E. amylovora DspE and DspE(Δβ-barrel) in Escherichia coli The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_ primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Campylobacter hyointestinalis Isolated from Pigs Produces Multiple Variants of Biologically Active Cytolethal Distending Toxin Article Snippet: The cdt-IIA, cdt-IIB, and cdt-IIC genes (open reading frames [ORFs] 11 to 13 in Fig. 2; also see Table S3 in the supplemental material) were PCR amplified using the genomic DNA of C. hyointestinalis strain ATCC 35217T as the template (see Table S1). .. Each of these amplified DNA fragments was digested with either EcoRI and HindIII or EcoRI and SacI (New England Biolabs Inc.) and cloned into the region downstream of the EcoRI site of the modified Article Title: The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo. Article Snippet: .. To construct plasmids for recombinant protein expression, DNA fragments encoding genes of interest were inserted into a modified Article Title: Structural characterization of NrnC identifies unifying features of dinucleases Article Snippet: For protein expression in Escherichia coli , codon-optimized NrnC genes from B. henselae (BH02530) and B. melitensis (BMEI1828) were synthesized by Geneart (Life Technologies). .. Genes were cloned into a modified Article Title: Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18 Article Snippet: Each of the DNA fragments encoding the human PTPN21 (residues 873-1174, referred to as PTP PTPN21 ) and human PTPN14 (residues 886-1187, referred to as PTP PTPN14 ) PTP domains were cloned into the modified pPRoEX HTa plasmid (Invitrogen, USA) with an N-terminal (His) 10 -linked green fluorescent protein tag and the pET21a plasmid (Novagen, USA), respectively. .. Each of the E7 DNA fragments encoding CR3s of HPV18 (residues 54-105, referred to as CR3 18E7 ), HPV16 (residues 45-98), HPV1a (residues 39-93), and HPV11 (residues 45-98) were cloned into the modified Article Title: Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster Article Snippet: .. The amplified fragments were then cloned into a modified Ubiquitin Proteomics:Article Title: Oligoribonuclease functions as a diribonucleotidase to bypass a key bottleneck in RNA degradation Article Snippet: Orn genes from Vibrio cholerae O1 El Tor VC0341 (residues 1-181) and Homo sapiens REXO2 (residues 33-237) were synthesized by Geneart (Life Technologies). .. Genes were cloned by ligation between BamHI and NotI sites of a modified Recombinant:Article Title: Oligoribonuclease functions as a diribonucleotidase to bypass a key bottleneck in RNA degradation Article Snippet: Orn genes from Vibrio cholerae O1 El Tor VC0341 (residues 1-181) and Homo sapiens REXO2 (residues 33-237) were synthesized by Geneart (Life Technologies). .. Genes were cloned by ligation between BamHI and NotI sites of a modified Article Title: The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo. Article Snippet: .. To construct plasmids for recombinant protein expression, DNA fragments encoding genes of interest were inserted into a modified Polymerase Chain Reaction:Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells. Article Snippet: Cloning, expression and purification of E. amylovora DspE and DspE(Δβ-barrel) in Escherichia coli The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_ primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells Article Snippet: The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Plasmid Preparation:Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells. Article Snippet: Cloning, expression and purification of E. amylovora DspE and DspE(Δβ-barrel) in Escherichia coli The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_ primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Structural characterization of NrnC identifies unifying features of dinucleases Article Snippet: For protein expression in Escherichia coli , codon-optimized NrnC genes from B. henselae (BH02530) and B. melitensis (BMEI1828) were synthesized by Geneart (Life Technologies). .. Genes were cloned into a modified Article Title: Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18 Article Snippet: Each of the DNA fragments encoding the human PTPN21 (residues 873-1174, referred to as PTP PTPN21 ) and human PTPN14 (residues 886-1187, referred to as PTP PTPN14 ) PTP domains were cloned into the modified pPRoEX HTa plasmid (Invitrogen, USA) with an N-terminal (His) 10 -linked green fluorescent protein tag and the pET21a plasmid (Novagen, USA), respectively. .. Each of the E7 DNA fragments encoding CR3s of HPV18 (residues 54-105, referred to as CR3 18E7 ), HPV16 (residues 45-98), HPV1a (residues 39-93), and HPV11 (residues 45-98) were cloned into the modified Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells Article Snippet: The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster Article Snippet: .. The amplified fragments were then cloned into a modified FLAG-tag:Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells. Article Snippet: Cloning, expression and purification of E. amylovora DspE and DspE(Δβ-barrel) in Escherichia coli The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_ primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Article Title: Bacterial pathogens deliver water- and solute-permeable channels to plant cells Article Snippet: The dspE gene was PCR-amplified from the genomic DNA of E. amylovora strain Ea273 using the KOD hot start polymerase (Millipore Sigma) and the following primer set: DspE_Forward_primer: 5′-ATGGAATTAAAATCACTGGGAACTGAACACAAG -3′; DspE_Reverse_primer: 5′-GCTCTTCATTTCCAGCCCTTCCTTC-3′. .. The PCR product was cloned into a modified Amplification:Article Title: Campylobacter hyointestinalis Isolated from Pigs Produces Multiple Variants of Biologically Active Cytolethal Distending Toxin Article Snippet: The cdt-IIA, cdt-IIB, and cdt-IIC genes (open reading frames [ORFs] 11 to 13 in Fig. 2; also see Table S3 in the supplemental material) were PCR amplified using the genomic DNA of C. hyointestinalis strain ATCC 35217T as the template (see Table S1). .. Each of these amplified DNA fragments was digested with either EcoRI and HindIII or EcoRI and SacI (New England Biolabs Inc.) and cloned into the region downstream of the EcoRI site of the modified Article Title: Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster Article Snippet: .. The amplified fragments were then cloned into a modified Construct:Article Title: The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo. Article Snippet: .. To construct plasmids for recombinant protein expression, DNA fragments encoding genes of interest were inserted into a modified Expressing:Article Title: The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo. Article Snippet: .. To construct plasmids for recombinant protein expression, DNA fragments encoding genes of interest were inserted into a modified Purification:Article Title: The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo. Article Snippet: .. To construct plasmids for recombinant protein expression, DNA fragments encoding genes of interest were inserted into a modified Cloning:Article Title: Structural characterization of NrnC identifies unifying features of dinucleases Article Snippet: For protein expression in Escherichia coli , codon-optimized NrnC genes from B. henselae (BH02530) and B. melitensis (BMEI1828) were synthesized by Geneart (Life Technologies). .. Genes were cloned into a modified Sequencing:Article Title: Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster Article Snippet: .. The amplified fragments were then cloned into a modified |